Decreased Erythrocyte Glyoxalase 1 (GLO1) Activity in Patients with Diabetes with Reduced Estimated Glomerular Filtration Rate

Sakly R1, Hamdi H1, Moussa A1, Lecube A2, Bouzidi H3, Zantour B4, Abid S1 and Kerkeni M1*

1Laboratory of Research on Biologically Compatible Compounds, University of Monastir, Tunisia
2Department of Endocrinology and Nutrition, University Hospital Arnau de Vilanova and Obesity, Diabetes and Metabolism (ODIM) Research Group, IRBL leida, University of Lleida, Spain
3Department of Biochemistry, CHU Tahar Sfar, Tunisia
4Department of Endocrinology, CHU Tahar Sfar, Tunisia

^*Correspondance to: Mohsen Kerkeni 

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Abstract:

Background: The glyoxalase enzymes are located in the cytosol of all cells, including erythrocytes, and prevent Advanced Glycation End products (AGEs) production through the detoxification of the Methylglyoxal (MGO). The present study was made to evaluate the GLO1 activity in diabetic patients and it relationship with estimated Glomerular Filtration Rate (eGFR). Patients and Methods: GLO1 activity was measured spectrophotometrically in erythrocytes of 123 participants: 35 healthy subjects and 88 patients with diabetes. Biochemical parameters were measured and eGFR was calculated using the MDRD (Modification of Diet in Renal Disease) formula. Results: We found no difference in GLO1 activity in patients with diabetes compared to healthy subjects. However GLO1 activity tended to be reduced in diabetic patients with loss renal function. A significant decrease was shown in patients with moderate to severe loss renal function. GLO1 activity was correlated with eGFR, creatinine and urea. Multivariate analysis showed that GLO1 activity was independently associated with eGFR. Conclusion: GLO1 activity was related with loss renal function in patients with diabetes according glomerular filtration rate.

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Cite the Article:

Sakly R, Hamdi H, Moussa A, Lecube A, Bouzidi H, Zantour B, et al. Decreased Erythrocyte Glyoxalase 1 (GLO1) Activity in Patients with Diabetes with Reduced Estimated Glomerular Filtration Rate. Ann Clin Case Rep. 2022; 7: 2263.