Case Report
Thymic Large-Cell Neuroendocrine Carcinoma: A Case Report and Review of a Japanese Case Series
Mariko Takishita1, Hisashi Saji1*, Hideki Marushima1, Tomoyuki Miyazawa1, Hiroyuki Kimura1,
Hiroki Sakai1, Yoichi Wakiyama1, Masataka Tsuda1, Koji Kojima1, Masahiro Hoshikawa2,
Masatomo Doi2, Masayuki Takagi2 and Haruhiko Nakamura1
1Department of Chest Surgery, St. Marianna University School of Medicine, Japan
2Department of Pathology, St. Marianna University School of Medicine, Japan
3Department of Thoracic Surgery, Tokyo Medical University, Japan
*Corresponding author: Hisashi Saji, Department of Chest Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamaeku, Kawasaki, Kanagawa 216-8511, Japan
Published: 21 Aug, 2018
Cite this article as: Takishita M, Saji H, Marushima H,
Miyazawa T, Kimura H, Sakai H, et al.
Thymic Large-Cell Neuroendocrine
Carcinoma: A Case Report and Review
of a Japanese Case Series. Ann Clin
Case Rep. 2018; 3: 1542.
Abstract
Large-Cell Neuroendocrine Carcinoma (LCNEC) of the thymus is a rare, aggressive cancer that leads
to a poorer prognosis than other thymic epithelial tumors. A 54 year old Chinese male presented with
an anterior Mediastinal mass as per chest computed tomography findings. Histological examination
after thymothymectomy revealed primary thymic LCNEC. We reviewed 40 cases of this rare cancer
that have been reported in Japan. To our knowledge, even though thymic LCNEC is sub classified as
part of the thymic NEC group in accordance with the classification of pulmonary neuroendocrine
tumors, the detailed clinical features and therapeutic management remain unknown.
Keywords: Large-cell neuroendocrine carcinoma; Thymic cancer; Prognosis
Introduction
Large-Cell Neuroendocrine Carcinoma (LCNEC) of the thymus is a rare malignant tumor that is more aggressive and leads to a poorer prognosis than other thymic epithelial tumors. Herein we describe a rare case of LCNEC of the thymus and review 40 cases of this disease that have been reported in Japan.
Case Report
A 54-year-old Chinese man was admitted to the St. Marianna University School of Medicine Hospital in October 2017 for further examination and treatment following the identification of an abnormal shadow in chest X-ray during a regular health checkup. The patient’s medical history included right renal infarction controlled by aspirin and hypertension treated with amlodipine and current smoking with 1.25 pack-year. The chest X-ray showed a solid mass with a clear border at the left hilum and an appearance of paravertebral body line (Figure1A). Enhanced chest Computed Tomography (CT) findings revealed a large, homogeneous, solid mass (3.5 cm) occupying the anterior mediastinum (Figure 1B). Magnetic Resonance Imaging (MRI) findings revealed that the tumor shared similar signal characteristics with the muscle on T1-weighted images. On T2-weighted images, the signal intensity was higher than that for muscle and revealed homogeneous enhancement. No signs of invasion of the aorta and pulmonary artery or metastases to other organs (Figure 1C and 1D) were noted. Fluoro-2-deoxyglucose positron emission tomography–computed tomography (FDG-PET/ CT) findings revealed the presence of an anterior Mediastinal tumor with high FDG accumulation with a standardized uptake value max (SUV max) of 5.3 (Figure 2A and 2B). Laboratory findings and results for tumor markers including CEA, CA125, CA19-9, SCC antigen, NSE, CYFRA, Pro-GRP, Alpha-fetoprotein, and IL-2 receptor were all within normal ranges preoperatively. Following the radiological diagnosis of thymoma, the patient underwent an extended thymothymectomy. Post median sternotomy, the thymus had macroscopically invaded the surrounding fatty tissue and adhered to the left pleura and the great vessels. Microscopically, the tumor had the morphologic features of a neuroendocrine tumor. The tumor cells were round-to-oval shaped and small foci of coagulation necrosis were observed (Figure 3A). Capsule invasion was also noted, but no invasive cancer cells existed in the connective tissue outside the capsule. Mitotic counts were >10 cells/10 HPF and the Ki-67 indices using MIB-1 immuno-histochemical staining ranged from 10% to 20% (Figure 2B). Immuno histochemistry findings revealed that the tumor cells strongly expressed Cytokeratin (CK) AE1/AE3, synaptophysin (Figure 2C), and CD56. Tumor cells were negative for CD5, CD20, P40, EMA, and p63 and were partially positive for chromogranin A (Figure 3D). The patient was finally pathologically diagnosed with LCNEC of the thymus, which was classified as a stage I tumor on the basis of Masaoka classification. The patient was treated using four courses of adjuvant chemotherapy on the basis of a platinum doublet using a cisplatin (80 mg/m2) and etoposide (100 mg/ m2) regimen. No recurrence was detected six months post-treatment.
Figure 1
Figure 1
Preoperative Chest X-ray and CT findings showed a solid mass with a clear border in the anterior
mediastinum (arrow) (A, B). Chest MRI showed that the tumor had signal characteristics similar to the muscle on
T1-weighted images. On T2-weighted images, the signal was higher than the muscle and revealed homogeneous
enhancement (C, D).
Figure 2
Figure 2
FDG-PET images reveal an anterior Mediastinal tumor with high
FDG accumulation with a standardized uptake value max (SUV max) of 9.9,
with no other abnormal FDG uptake, including the testes (A, B).
Figure 3
Figure 3
Microscopy findings showed that the tumor cells were round-tooval
shaped with small foci of coagulation necrosis (A). Mitotic counts were
>10 cells/10 HPF, and the Ki-67 indices using MIB-1 immuno-histochemical
staining ranged from 10% to 20% (B). Immuno-histochemical findings
showed that the tumor cells strongly expressed synaptophysin (C) and
chromogranin A (D).
Table 1
Discussion
Thymic NECs are relatively rare neoplasms that account for 2% to
4% of all anterior Mediastinal neoplasms [1]. Primary thymic LCNEC
is a high-grade malignant thymic tumor that comprises large cells
with neuroendocrine morphology and either neurosecretory granules
as observed on electron microscopy or positive neuroendocrine
immuno-histochemical markers [2]. Even though LCNEC is sub
classified as part of the thymic NEC group in accordance with the
classification of pulmonary neuroendocrine tumors, the detailed clinical features of LCNEDC remain unknown. Fourty cases,
including the present case, of primary thymic LCNEC have been
reported until 2017 in the Japanese literature (Table 1). The average
age of patients was 53.7 years (range 30-75 years). Patients included 17
men, 21 women, and 2 patients with an unshared gender. In general,
thymic neuroendocrine tumors (NETs) occurred in patients who
were approximately 50 years of age [3]. Thymic LCNEC is a highgrade
malignant tumor composed of large cells with neuroendocrine
features. The high-grade tumors (SCNEC and LCNEC) are less
common in men compared with the carcinoid tumor. Therefore, the
present case can be considered as relatively rare.
The symptoms of thymic cancer may be associated with the tumor
itself or be part of a condition related to the cancer. However, they
are not directly caused by the tumor mass. Examples of symptoms
include shortness of breath, cough, chest pain, and swelling of the
face. Some patients with thymic LCNEC do not show any symptoms
and are identified by an abnormal shadow on the chest X-ray during
a regular checkup. Regardless of the presence of symptoms, thymic
LCNEC is more aggressive than other thymic epithelial tumors and
LCNECs present primarily during advanced clinical stages. At this
stage, patients already have an advanced disease stage or metastatic
tumors. Detailed clinical features, long-term prognosis, and definitive
modalities of treatment for LCNEC of the thymus remain unknown.
Studies have reported that the clinical behavior and prognosis of lung
LCNEC are similar to Small Cell Lung Cancer (SCLC). SCLC-based
regimens are considered to be effective in patients with LCNEC.
Therefore, postoperative adjuvant chemotherapy consisting CDDP
and VP-16 is recommended, which is the treatment protocol for
SCLC. We believe that the regimen may be effective in cases of thymic
LCNEC, which has a poorer prognosis than other neuroendocrine
tumors because of the frequent distant and lymph node metastases.
Four cancer-related deaths occurred during follow-up, 14 patients
had recurrence including metastases. The median survival time was
14 months in our Japanese series. Shoji et al. reviewed 13 cases of
thymic LCNECs worldwide [4]. The five-year overall survival and
five-year disease-free survival rates were 66% and 43%, respectively.
In the present case, the potential risk of recurrence necessitates
regular follow-up.
Acknowledgement
Medical English writing assistance was provided by Crimson Interactive Pvt. Ltd. The authors are fully responsible for the content and editorial decisions regarding this manuscript.
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